Origin pairing (‘handcuffing’) as a mode of negative control of P1 plasmid copy number

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A handcuff model for the cohesin complex

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The shooter who killed 22 people in Nova Scotia began his attack by handcuffing and assaulting his girlfriend Saturday night before she.

The cohesin complex is responsible for the accurate separation of sister chromatids into two daughter cells. Several models for the cohesin complex have been proposed, but the one-ring embrace model currently predominates the field. However, the static configuration of the embrace model is not flexible enough for cohesins to perform their functions during DNA replication, transcription, and DNA repair. We used coimmunoprecipitation, a protein fragment complement assay, and a yeast two-hybrid assay to analyze the protein—protein interactions among cohesin subunits.

These data support a two-ring handcuff model for the cohesin complex, which is flexible enough to establish and maintain sister chromatid cohesion as well as ensure the fidelity of chromosome segregation in higher eukaryotes. An evolutionarily conserved protein complex called cohesin is responsible for the accurate separation of sister chromatids into two daughter cells. The cohesin complex comprises four core protein subunits that are conserved from yeast to vertebrates Guacci et al.

The amino terminus NT and carboxyl terminus CT of the Smc molecules fold back on themselves, forming antiparallel intramolecular coiled coils Haering et al.

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We might like to think that we are deeply complex creatures, but when it comes down to it, we are actually pretty simple. In the summer, when the sun is shining and the temperature is warm, we are more content to remain single. We socialise more, meet new people, and enjoy the feeling of being untethered. As the leaves turn from green to crispy shades of Autumn however, we yearn for companionship.

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WaPo editorial criticizes Pompeo. Test your knowledge of strange human behaviors. With our free Puku Summer Camp! Just a shot in the dark here. Cuffing season refers to a period of time where single people begin looking for short term partnerships to pass the colder months of the year. Cuffing season usually begins in October and lasts until just after Valentines Day.

The use of the word cuff references handcuffs, but is slang in the same vein as “hooking up” or “getting hitched. It makes sense, then, for cuffing season to be impacted by the weather. It seems like the change in seasons are only denoted by the date now. It is cold into spring, it is warm well into fall. How could cuffing season survive when the true seasons of fall and winter are not?

N.S. shooter handcuffed, assaulted his girlfriend before mass killing

Syracuse, NY — A civilian employee of the Syracuse police department has been charged with forcibly touching his girlfriend after handcuffing her, according to a criminal complaint. Brett Forkhamer, 29, was arrested Monday in connection with the incident, which was among a string of complaints made by the ex-girlfriend against him. Forcible touching is a misdemeanor punishable by up to a year in jail.

The woman accused him of repeatedly handcuffing, then committing sexual acts against her, including slapping her and touching her, she told police. The alleged crime appears to arise from another allegation:.

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Top definition. Cuffing Season. Charlotte: Ugh no. Aug 18 Word of the Day. Are they a girl or a boy? Neither , they’re an enby!

The use of the word cuff references handcuffs, but is slang in the same vein as “​hooking The earliest recorded print uses of cuffing season date from college.

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The most popular dating sites in the UK, just in time for cuffing season to be “​cuffed” by a serious relationship, is known as cuffing season.

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